EC Number |
Protein Variants |
Reference |
---|
4.2.1.115 | C103M |
site-directed mutagenesis, the mutant is inactive, dimerization is prevented but the secondary structure is not significantly affected |
692961 |
4.2.1.115 | C118M |
site-directed mutagenesis, not recombinantly expressable mutant |
692961 |
4.2.1.115 | D126N |
site-directed mutagenesis, the mutant shows about 100fold lower activity with UDP-GlcNAc and with UDP-6-deoxy-6-fluoro-GlcNAc for HF elimination compared to the wild-type enzyme. Upon addition of UDP-4-keto-6-deoxy-GlcNAc to D126N the tightly bound NADPH is immediately oxidized |
691202 |
4.2.1.115 | D149K/K150A |
site-directed mutagenesis, the mutant is inactive |
692961 |
4.2.1.115 | D149K/K150D |
site-directed mutagenesis, the mutant is inactive |
692961 |
4.2.1.115 | D70A |
site-directed mutagenesis, the mutant is inactive |
692961 |
4.2.1.115 | D70N |
site-directed mutagenesis, not recombinantly expressable mutant |
692961 |
4.2.1.115 | F78W |
site-directed mutagenesis of a cofactor binding residue, the mutant shows similar activity compared to the wild-type enzyme |
729735 |
4.2.1.115 | G20A |
site-directed mutagenesis, the mutant is inactive |
692961 |
4.2.1.115 | H86A |
site-directed mutagenesis, insoluble protein, the mutant shows reduced activity compared to the wild-type enzyme |
692961 |