EC Number |
Protein Variants |
Reference |
---|
4.1.99.22 | R192A |
80% loss of activity |
716738 |
4.1.99.22 | R71A |
80% loss of activity |
716738 |
4.1.99.22 | R17A |
complete loss of activity |
716738 |
4.1.99.22 | R17A/R266A/R268A |
complete loss of activity |
716738 |
4.1.99.22 | R266A |
complete loss of activity |
716738 |
4.1.99.22 | R268A |
complete loss of activity |
716738 |
4.1.99.22 | more |
deletion of central components of the ISC system in addition to IscS leads to an overall decrease in Fe-S cluster enzyme and molybdoenzyme activity in addition to a decrease in the number of Fe-S-dependent thiomodifications of tRNA, based on the fact that some proteins involved in Moco biosynthesis and tRNA thiolation are Fe-S-dependent. Complementation of the ISC deficient strains with the suf operon restores the activity of Fe-S-containing proteins, including the MoaA protein, which is involved in the conversion of 5'-GTP to cyclic pyranopterin monophosphate in the fist step of Moco biosynthesis |
-, 747120 |
4.1.99.22 | more |
generation of cnx2 mutants by T-DNA insertion, line cnx2-1, and point mutation, line cnx2-2. Mutation mapping by whole genome sequencing, phenotype analyses |
-, 747034 |
4.1.99.22 | S126A |
mutant enzyme with low activity |
716738 |
4.1.99.22 | T73A |
mutant enzyme with low activity |
716738 |