EC Number   |
Protein Variants |
Reference |
|---|
   3.8.1.9 | D205E |
activity with D-2-chloropropionate is 10.1% compared to activity of the wild-type enzyme. The kcat value of wild-type enzyme is 13fold higher compared to mutant enzyme |
758426 |
| 3.8.1.9 | D205N |
catalytically inactive variant |
756727 |
| 3.8.1.9 | D205N |
no activity with D-2-chloropropionate |
-, 758426 |
| 3.8.1.9 | F281A |
activity with D-2-chloropropionate is 54.7% compared to activity of the wild-type enzyme |
758426 |
| 3.8.1.9 | G50A |
activity with D-2-chloropropionate is 5.1% compared to activity of the wild-type enzyme |
-, 758426 |
| 3.8.1.9 | I52G |
activity with D-2-chloropropionate is 1.5% compared to activity of the wild-type enzyme |
758426 |
| 3.8.1.9 | L285I |
activity with D-2-chloropropionate is 24.4% compared to activity of the wild-type enzyme |
758426 |
| 3.8.1.9 | L288A |
the mutant enzyme is active toward L-enantiomers. The catalytic efficiency is less than 30% of mutant enzyme L288I |
756727 |
| 3.8.1.9 | L288I |
activity with D-2-chloropropionate is 37.2% compared to activity of the wild-type enzyme |
758426 |
| 3.8.1.9 | L288I |
the mutation enlarges the size of the channel and allows the enzyme to accommodate L-enantiomers (L-2-bromopropionate). The wing flip of I288 induces hydrophobic interactions with the L-enantiomer and directly affects the catalytic efficiency |
756727 |
