EC Number   |
Protein Variants |
Reference |
|---|
   3.6.4.B6 | D290A |
mutation has no effect on ATP hydrolysis |
-, 724227 |
| 3.6.4.B6 | DELTA1-224 |
in strains expressing the mutant enzyme no archaellum is assembled and swimming motility is abolished. Mutant enzyme still exhibits 75% of ATPase activity compared to the full-length FlaI |
-, 725972 |
| 3.6.4.B6 | DELTA1-29 |
strains overexpressing FlaIDELTA129 show that 10%20% of the cell population can assemble archaella, whereas complementation with wild-type FlaI leads to 40%50% of cells having archaella |
-, 725972 |
| 3.6.4.B6 | E336A |
mutation results in a reduction of approximately 90% of ATP hydrolysis compared with wild-type enzyme, mutant forms a stable oligomer after ATP binding |
-, 724227 |
| 3.6.4.B6 | K268A |
mutation results in a reduction of approximately 50% of ATP hydrolysis compared with wild-type enzyme, 20-fold lower binding affinity of ATP, no oligomerization |
-, 724227 |
| 3.6.4.B6 | M69E/I72E/F76E |
localization to the membrane is significantly reduced compared to that of wild-type |
-, 725972 |
| 3.6.4.B6 | more |
construction of various truncated versions of FlaI and interaction analysis with the archaellum subunit FlaX |
-, 733831 |
