EC Number   |
Protein Variants |
Reference |
|---|
    3.5.4.27 | E186A |
Vmax is 0.035% of wild-type activity, Km for 5,10-methenyl-5,6,7,8-tetrahydromethanopterin is 2.1fold lower compared to wild-type value |
721676 |
| 3.5.4.27 | E186D |
Vmax is 0.56% of wild-type activity, Km for 5,10-methenyl-5,6,7,8-tetrahydromethanopterin is 1.1fold higher compared to wild-type value |
721676 |
| 3.5.4.27 | E186N |
Vmax is 0.1% of wild-type activity, Km for 5,10-methenyl-5,6,7,8-tetrahydromethanopterin is 1.1fold lower compared to wild-type value |
721676 |
| 3.5.4.27 | E186Q |
nearly inactive mutant enhzyme |
721676 |
| 3.5.4.27 | F190V |
no activity |
721676 |
| 3.5.4.27 | K94E |
Vmax is 86% of wild-type activity, Km for 5,10-methenyl-5,6,7,8-tetrahydromethanopterin is 1.4fold lower compared to wild-type value |
721676 |
| 3.5.4.27 | K94V |
Vmax is 41% of wild-type activity, Km for 5,10-methenyl-5,6,7,8-tetrahydromethanopterin is 1.2fold lower compared to wild-type value |
721676 |
| 3.5.4.27 | R183a |
Vmax is 0.07% of wild-type activity, Km for 5,10-methenyl-5,6,7,8-tetrahydromethanopterin is 1.3fold lower compared to wild-type value |
721676 |
| 3.5.4.27 | R183E |
Vmax is 0.01% of wild-type activity, Km for 5,10-methenyl-5,6,7,8-tetrahydromethanopterin is 2.2fold lower compared to wild-type value |
721676 |
| 3.5.4.27 | R183E/E186Q |
no activity |
721676 |
