EC Number   |
Protein Variants |
Reference |
|---|
   3.4.23.39 | D158A |
active-site mutant of both plasmepsin II and IV shows neither autocatalytic processing nor proteolytic activities |
669157 |
| 3.4.23.39 | D214A |
mutation abolishes proteolytic activity |
680725 |
| 3.4.23.39 | D338A |
active-site mutant of both plasmepsin II and IV shows neither autocatalytic processing nor proteolytic activities |
669157 |
| 3.4.23.39 | D34A |
mutation abolishes proteolytic activity |
680725 |
| 3.4.23.39 | D34S |
mutation abolishes proteolytic activity |
680725 |
| 3.4.23.39 | D34S/D214S |
mutation abolishes proteolytic activity |
680725 |
| 3.4.23.39 | I287E |
changes in substrate specificity compared to wild-type enzyme, the turnover-number for Ala-Leu-Glu-Arg-Nle-Phe-Phe(NO2)-Ser-Phe-Pro is 42% of that of the wild-type enzyme, the Km-value is 51% of that of the wild-type enzyme |
648206 |
| 3.4.23.39 | M13E |
the turnover-number for Ala-Leu-Glu-Arg-Nle-Phe-Phe(NO2)-Ser-Phe-Pro is 1.16fold higher than the turnover-number of the wild-type enzyme, the Km-value is 54% of that of the wild-type enzyme |
648206 |
| 3.4.23.39 | M13E/I287E |
changes in substrate specificity compared to wild-type enzyme, the turnover-number for Ala-Leu-Glu-Arg-Nle-Phe-Phe(NO2)-Ser-Phe-Pro is 1.48fold higher than the turnover-number of the wild-type enzyme, the Km-value is 1.23fold higher than that of the wild-type enzyme |
648206 |
| 3.4.23.39 | M205S |
mutant enzyme with reduced chances of autolysis during purification |
648204 |
