EC Number |
Protein Variants |
Reference |
---|
3.4.22.52 | C115A |
non-autolysing active-site mutant |
667872 |
3.4.22.52 | C115S |
mutant without proteolytic activity of autolysis and caseinolysis |
643978 |
3.4.22.52 | H272A |
catalytically inactive |
710164 |
3.4.22.52 | more |
- |
643967 |
3.4.22.52 | more |
polymorphisms in the calpain 1 gene influence meat quality in carcass trait |
717053 |
3.4.22.52 | more |
the mu-calpain single nucleotide polymorphisms C80T, C302G, G310A, C445T, and A524C are associated with tenderness in beef cattle |
707143 |
3.4.22.52 | more |
the mutant enzyme L-muyCANPDELTA3 requires 0.4-0.53 mM of Ca2+ compared to 0.06 mM for the native enzyme, bacterially expressed mutant enzyme L-muyCANPDELTA3. A chimeric form composed of domains I-III of muCANP and domain IV of calpain II is also expressed in Sf9 cells. This mutant requires less Ca2+, 0.05 mM, than the native erythrocyte enzyme and has the highest specific activity of all calpains tested. All recombinant proteins are active as monomers in polyethylene glycol-containing buffers. The in vitro association with the regulatory subunit enhances only slightly the maximal velocity and the Ca2+ dependence of the expressed proteins |
643976 |