EC Number   |
Protein Variants |
Reference |
|---|
   3.4.21.B48 | E78H |
0.01% activity compared to wild-type enzyme, the canonical catalytic triad is disrupted |
-, 652534 |
| 3.4.21.B48 | S278A |
an inactive pro-kumamolisin mutant, the catalytic domain of the mutant exhibits a virtually identical structure compared to the active enzyme |
-, 731647 |
| 3.4.21.B48 | D164A |
inactive |
718028 |
| 3.4.21.B48 | more |
mutational analysis of the Ser278 residue reveals that the mutant loses both auto-processing activity and proteolytic activity |
-, 651795 |
| 3.4.21.B48 | D316A |
site-directed mutagenesis, autoactivation similar to the wild-type enzyme, 3% of wild-type enzyme activity |
-, 665094 |
| 3.4.21.B48 | E78A/D164N |
site-directed mutagenesis, completely inactive mutant which stays unprocessed |
-, 664913 |
| 3.4.21.B48 | E78Q/D164N |
site-directed mutagenesis, completely inactive mutant which stays unprocessed |
-, 664913 |
| 3.4.21.B48 | D164A |
site-directed mutagenesis, no autoactivation of the proform, inactive mutant |
-, 665094 |
| 3.4.21.B48 | D82A |
site-directed mutagenesis, no autoactivation of the proform, inactive mutant |
-, 665094 |
| 3.4.21.B48 | E78A |
site-directed mutagenesis, no autoactivation of the proform, inactive mutant |
-, 665094 |
