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EC Number
Protein Variants
Commentary
Reference
3.4.21.89
C51A
Lbpro mutant, site-directed mutagenesis
137299
3.4.21.89
C51A/C133S
Lbpro-double mutant, site-directed mutagenesis
137299
3.4.21.89
D142E
10% of wild-type activity
669425
3.4.21.89
D142I
as active as wild-type enzyme
669425
3.4.21.89
D187A
no catalytic activity
-
,
727546
3.4.21.89
D187N
no catalytic activity
-
,
727546
3.4.21.89
D215A
Vmax is 1.8fold lower than wild-type value, kcat is 1.8 fold lower than wild-type enzyme
669435
3.4.21.89
D273A
site directed scanning mutagenesis
652083
3.4.21.89
D273A/R146A
site directed scanning mutagenesis
652083
3.4.21.89
D273A/R146A/T94V
site directed scanning mutagenesis
652083
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