EC Number   |
Protein Variants |
Reference |
|---|
   3.2.1.81 | E147S |
inactive mutant |
-, 670907 |
| 3.2.1.81 | E99K |
the mutant shows 140% relative activity compared to the wild type enzyme |
-, 702840 |
| 3.2.1.81 | E99K/T307I |
the mutant shows 200% relative activity compared to the wild type enzyme |
-, 702840 |
| 3.2.1.81 | L122Q |
shows similar thermostability with wild-type AgaB, raises specific activity 1.3fold |
695671 |
| 3.2.1.81 | L122Q/N446I |
has higher thermostability and slightly increased specific activity (1.1fold) than wild-type AgaB. Melting temperature of S2 is 4.6°C higher than that of wild-type AgaB, and the half-life of S2 is 350 min at 40°C, which is 18.4fold longer than that of the wild-type enzyme. Comparable pH stability and optimum pH and temperature profiles as the wild-type |
695671 |
| 3.2.1.81 | more |
construction of two C-terminally truncated forms of enzyme lacking 127 and 182 amino acids, the protein lacking 127 amino acids has a lower Km than wild type and higher thermal stability at 50°C |
-, 656476 |
| 3.2.1.81 | more |
expression in Escherichia coli of full-length protein, of the catalytic module, and of the carbohydrate binding module. The catalytic module alone exhibits 50fold higher acivity than the full-length enzyme |
678815 |
| 3.2.1.81 | more |
inactive mutant A-E147S, crystallization data |
657375 |
| 3.2.1.81 | N103T |
the mutant shows specific activity similar to the wild type enzyme |
702825 |
| 3.2.1.81 | N446I |
has similar enhanced thermostability as mutant L122Q/N446I, and decreased specific activity by 10-20% as compared to the wild-type |
695671 |
