EC Number   |
Protein Variants |
Reference |
|---|
    3.2.1.68 | more |
Cassava starch is debranched by treatment with isoamylase and pullulanase and the yield of resistant starch type III, method optimization with respect to starch solids concentration, incubation time, and enzyme concentration using central composite rotatable design |
710571 |
| 3.2.1.68 | more |
construction of a single mutant line isa2-2 by T-DNA insertion mutagenesis. The isa1-1 isa2-2 double mutant line is obtained by a cross between the two corresponding single mutants and screening of the progeny by PCR amplification of genomic DNA using a T-DNA end primer and gene-specific primers. The phenotype of the isa1-1 isa2-2 double mutant is complemented but the ZmISA1 activity |
-, 732655 |
| 3.2.1.68 | more |
construction of single mutant line isa1-1 by T-DNA insertion mutagenesis. The isa1-1 isa2-2 double mutant line is obtained by a cross between the two corresponding single mutants and screening of the progeny by PCR amplification of genomic DNA using a T-DNA end primer and gene-specific primers. The phenotype of the isa1-1 isa2-2 double mutant is complemented but the ZmISA1 activity, Zea mays ISA1 subunit is expressed via transfection with Agrobacterium tumefaciens strain GV3101 in Arabidopsis thaliana lacking endogenous ISA1 or lacking both native ISA1 and ISA2. The maize protein functions in Arabidopsis leaves to support nearly normal starch metabolism in the absence of any native ISA1 or ISA2 |
-, 732655 |
| 3.2.1.68 | more |
functional complementation of sta8 mutant strains BafV13 and BafO6 with ISA2 or ISA2-HA, overview |
-, 732146 |
| 3.2.1.68 | more |
generation of mutant isa2-339 |
732581 |
| 3.2.1.68 | more |
generation of mutant su1-4582 |
732581 |
| 3.2.1.68 | more |
mutant sta8, about 35% residual enzymic activity, loss of two out of three activity bands in zymogram gels, loss of about 50% of mass of the multimeric complex |
657032 |
| 3.2.1.68 | more |
mutations in the N-terminal region result in a sharp increase in alpha-1,4-transferase activity and a reduced level of alpha-1,6-glucosidase activity, overview |
693147 |
| 3.2.1.68 | D367A |
site-directed mutagenesis of the catalytic nucleophile Asp-367 in ISA1, the mutation abolishes the catalytic activity of the enzyme complex |
732750 |
| 3.2.1.68 | R505E |
the acidic stability is enhanced and the specific activity of the mutant is increased by 13% compared to the wild type enzyme |
-, 751254 |
