EC Number   |
Protein Variants |
Reference |
|---|
   3.2.1.203 | D468A |
calcium binding-site mutant, shows increased activity compared with the wild type with 0 mM or 10 mM Ca2+ added. The mutation triggers a conformational change similar to that induced by Ca2+ in the wild type. Mutant displays an increase in melting temperature by 5.9°C |
735511 |
| 3.2.1.203 | D468E |
calcium binding-site mutant, kinetic parameters similar to wild-type |
735511 |
| 3.2.1.203 | D468V |
calcium binding-site mutant, shows increased activity compared with the wild type with 0 mM or 10 mM Ca2+ added. Mutant displays an increase in melting temperature by 4.5°C |
735511 |
| 3.2.1.203 | D469A |
calcium binding-site mutant, results in decreased activity |
735511 |
| 3.2.1.203 | D469V |
calcium binding-site mutant, increase in kcat value |
735511 |
| 3.2.1.203 | D471A |
calcium binding-site mutant, results in decreased activity |
735511 |
| 3.2.1.203 | D471E |
calcium binding-site mutant, kinetic parameters similar to wild-type |
735511 |
| 3.2.1.203 | D471V |
calcium binding-site mutant, increase in km value |
735511 |
| 3.2.1.203 | F16S |
1.7fold increase in kcat and 1.5fold improvement in hydrolytic activity on cellulosic substrates, while maintaining thermostability |
-, 742136 |
| 3.2.1.203 | F16S/Y95F |
60% reduction in catalytic efficiency |
-, 742136 |
