EC Number   |
Protein Variants |
Reference |
|---|
    3.2.1.179 | D115N |
site-directed mutagenesis, the mutant shows only negligible enzyme activity |
755323 |
| 3.2.1.179 | D115N/K370S |
site-directed mutagenesis, the mutant shows only negligible enzyme activity |
755323 |
| 3.2.1.179 | D149N |
almost inactive mutant enzyme, kcat/Km is significantly lower than that of the wild-type enzyme for gellan tetrasaccharide |
-, 708980 |
| 3.2.1.179 | D88N |
almost inactive mutant enzyme, kcat/Km is significantly lower than that of the wild-type enzyme for gellan tetrasaccharide |
-, 708980 |
| 3.2.1.179 | G342S |
kcat/KM for beta-D-4-deoxy-DELTA4,5-GlcAp-(1->3)-beta-D-GalNAc6S is similar to the wild-type value, kcat/Km for beta-D-4-deoxy-DELTA4,5-GlcAp-(1->3)-beta-D-GalNAc is similar to wild-type value |
-, 709164 |
| 3.2.1.179 | H339S |
kcat/KM for beta-D-4-deoxy-DELTA4,5-GlcAp-(1->3)-beta-D-GalNAc6S is 1.2fold higher than the kcat/Km-value for the wild-type enzyme, kcat/Km for beta-D-4-deoxy-DELTA4,5-GlcAp-(1->3)-beta-D-GalNAc is 53% of the wild-type value |
-, 709164 |
| 3.2.1.179 | I344K |
kcat/KM for beta-D-4-deoxy-DELTA4,5-GlcAp-(1->3)-beta-D-GalNAc6S is 6.1fold higher than the wild-type value, kcat/Km for beta-D-4-deoxy-DELTA4,5-GlcAp-(1->3)-beta-D-GalNAc is similar to the wild-type value |
-, 709164 |
