EC Number |
Protein Variants |
Reference |
---|
3.2.1.140 | more |
truncated enzyme without N-terminal signal peptide of 34 amino acids and C-terminal membrane anchor from 1065-1108 |
695742 |
3.2.1.140 | D320A |
site-directed mutagenesis, the mutant shows significantly reduced kcat, but unaffected Km compared with the wild-type enzyme |
732092 |
3.2.1.140 | D320N |
site-directed mutagenesis, the mutant shows significantly reduced kcat, but unaffected Km compared with the wild-type enzyme |
732092 |
3.2.1.140 | H263F |
site-directed mutagenesis, the mutant shows significantly reduced kcat compared with the wild-type enzyme |
732092 |
3.2.1.140 | Y419F |
site-directed mutagenesis, the mutant shows significantly reduced Km and kcat values compared with the wild-type enzyme |
732092 |
3.2.1.140 | more |
generation of different truncated forms of the enzyme, structure-function analysis in comparison to the wild-type, overview. The large Bifidobacterium bifidum lacto-N-biosidase and its truncated mutants are used as a model proteins to evaluate the minimal functional unit due to its interest and structural complexity |
755088 |