EC Number   |
Protein Variants |
Reference |
|---|
   3.1.1.96 | E137A |
in presence of 0.01 mM Zn2+ and 4 mM MgCl2 the enzyme displays an activity 10fold lower than that of the wild type. The mutant enzyme contains onyl one Zn2+ |
725371 |
| 3.1.1.96 | E154A |
in presence of 0.01 mM Zn2+ and 4 mM MgCl2 mutation reduces activity. The mutant enzyme contains onyl one Zn2+ |
725371 |
| 3.1.1.96 | H140A |
in presence of 0.01 mM Zn2+ and 4 mM MgCl2 activity of the H140A variant is so small that it can not be detected, even with 70 nM enzyme in the assay |
725371 |
| 3.1.1.96 | H141A |
in presence of 0.01 mM Zn2+ and 4 mM MgCl2 variant exhibits the same activity as that of the wild-type protein |
725371 |
| 3.1.1.96 | H197A |
in presence of 0.01 mM Zn2+ and 4 mM MgCl2 mutation increases activity |
725371 |
| 3.1.1.96 | H80A |
in presence of 0.01 mM Zn2+ and 4 mM MgCl2 mutation reduces activity. The mutant enzyme contains onyl one Zn2+ |
725371 |
| 3.1.1.96 | H92A |
in presence of 0.01 mM Zn2+ and 4 mM MgCl2 mutation reduces activity |
725371 |
| 3.1.1.96 | S78A |
in presence of 0.01 mM Zn2+ and 4 mM MgCl2 mutation reduces activity |
725371 |
| 3.1.1.96 | up |
Co2+, Mn2+, and Ni2+ markedly contribute to the expression of the deacylase activity |
725386 |
| 3.1.1.96 | W162A |
in presence of 0.01 mM Zn2+ and 4 mM MgCl2 mutation increases activity |
725371 |
