EC Number   |
Protein Variants |
Reference |
|---|
   2.8.2.25 | H118A |
strong reduction of catalytic activity |
644312 |
| 2.8.2.25 | H118E |
strong reduction of catalytic activity |
644312 |
| 2.8.2.25 | K59A |
300fold decrease in specific activity, K59 is not required for cosubstrate binding |
644313 |
| 2.8.2.25 | K59R |
15-fold decrease in specific activity, K59 is not required for cosubstrate binding |
644315 |
| 2.8.2.25 | K59R |
K59 interacts with 5-portion of the nucleotide, involved in proper orientation of the phosphosulfate group |
644313 |
| 2.8.2.25 | L95Y |
different effects on kinetic konstants |
644312 |
| 2.8.2.25 | more |
construction of chimeric enzymes with EC2.8.2.27, to find domains defining substrate and position specificity |
644316 |
| 2.8.2.25 | more |
kinetic data of several mutant enzymes, unconservative mutations in K134, Y137 or Y150 lead to protein instability in solution |
644312 |
| 2.8.2.25 | R140K |
no effect on protein stability, strong reduction of specific activity |
644312 |
| 2.8.2.25 | R140S |
no effect on protein stability, strong reduction of specific activity |
644312 |
