EC Number   |
Protein Variants |
Reference |
|---|
   2.7.7.48 | A105S |
62% activity compared to the wild type enzyme |
693122 |
| 2.7.7.48 | A185V |
mutation causes change in secondary structure |
759905 |
| 2.7.7.48 | A365N |
mutation selected to stabilize the secondary structural elements near the rNTP binding pocket of the enzyme. Mutant viruses were tested in vitro on Vero, C6/36, Culex tarsalis and DF-1 cell types and in vivo in one day old chickens and Culex pipiens mosquitoes. Mutation affects plaque morphology and particularly alters growth and RNA replication kinetics |
-, 723799 |
| 2.7.7.48 | A396G |
the mutation occurs under PSI-6130 selection in cell culture, it reduces the enzyme activity compared to the wild-type enzyme |
701728 |
| 2.7.7.48 | A421V |
the mutation occurs under PSI-6130 selection in cell culture, it reduces the enzyme activity compared to the wild-type enzyme |
701728 |
| 2.7.7.48 | A466V |
mutation has no effect on secondary structure |
759905 |
| 2.7.7.48 | A97S |
88% activity compared to the wild type enzyme |
693122 |
| 2.7.7.48 | A97V |
mutation causes change in secondary structure |
759905 |
| 2.7.7.48 | C274A |
mutation completely abolishes RNA-dependent RNA polymerase activity |
643389 |
| 2.7.7.48 | C575S |
the mutation occurs under PSI-6130 selection in cell culture, it reduces the enzyme activity compared to the wild-type enzyme |
701728 |
