EC Number   |
Protein Variants |
Reference |
|---|
   2.7.7.37 | H308A |
site-directed mutagenesis |
729696 |
| 2.7.7.37 | K337A |
site-directed mutagenesis |
729696 |
| 2.7.7.37 | K340A |
site-directed mutagenesis |
729696 |
| 2.7.7.37 | more |
construction of expression vectors encoding a series of ST0452 C-terminal deletion mutants with hexahistidine tags at their C-termini, designated pST0452(DC005)H, pST0452(DC011)H, pST0452(DC021)H, pST0452(DC031)H, pST0452(DC041) H, pST0452(DC051)H, pST0452(DC071)H, pST0452 (DC121)H and pST0452(DC171)H. The deletion mutants retain the same tertiary structures as the wild-type ST0452 protein |
729696 |
| 2.7.7.37 | more |
construction of several truncated mutants of the PH0925 protein, that show altered kinetics compared to the wild-type enzyme, overview |
-, 733811 |
| 2.7.7.37 | more |
site-directed mutagenesis of the active site results in 11 mutants, five of which sows increased kcat and Km values, but also increased activity compared to the wild-type, all mutants show the same thermostability as the wild-type |
-, 739829 |
| 2.7.7.37 | N331A |
site-directed mutagenesis |
729696 |
| 2.7.7.37 | Y311A |
site-directed mutagenesis |
729696 |
