EC Number   |
Protein Variants |
Reference |
|---|
    2.7.4.13 | more |
all enzyme mutants but T17S show an increased pH optimum compared to the wild-type enzyme |
739645 |
| 2.7.4.13 | W150F |
site-directed mutagenesis of the conserved residue, the mutant shows unaltered activity compared to the wild-type enzyme |
739645 |
| 2.7.4.13 | W150A |
site-directed mutagenesis of the core residue |
739645 |
| 2.7.4.13 | D170N |
site-directed mutagenesis of the core residue, almost inactive mutant |
739645 |
| 2.7.4.13 | E176Q |
site-directed mutagenesis of the core residue, almost inactive mutant |
739645 |
| 2.7.4.13 | R172I |
site-directed mutagenesis of the core residue, almost inactive mutant |
739645 |
| 2.7.4.13 | R130K |
site-directed mutagenesis of the dNMP-binding residue, almost inactive mutant |
739645 |
| 2.7.4.13 | G137A |
site-directed mutagenesis of the dNMP-binding residue, the mutant shows highly reduced activity and altered substrate specificity compared to the wild-type enzyme |
739645 |
| 2.7.4.13 | Q134A |
site-directed mutagenesis of the dNMP-binding residue, the mutant shows reduced activity and altered substrate specificity compared to the wild-type enzyme |
739645 |
| 2.7.4.13 | T138A |
site-directed mutagenesis of the dNMP-binding residue, the mutant shows reduced activity and altered substrate specificity compared to the wild-type enzyme |
739645 |
