EC Number   |
Protein Variants |
Reference |
|---|
   2.7.1.46 | E564K |
a ara2-1 mutant form of the enzyme that carries a point mutation in an alpha-helix. The mutation is close to the substrate binding site. The residual enzymatic activity of ARAK2 mutant E564K is below 1% when compared with the wild-type ARAK2 variant. 227fold increase of Km value for L-arabinose for ARA2 E564K mutant compared to wild-type |
759961 |
| 2.7.1.46 | E655K |
an ara1-1 mutant form of the enzyme that carries a point mutation in an alpha-helix. Almost inactive mutant. The mutation is close to the substrate binding site and changes the Km value for arabinose from 0.08 mM in the wild-type to 17 mM in ARA1-1 mutant. Analysis of marker genes from sugar signalling pathways (SnRK1 and Tor) suggests that ara1-1 misinterprets its carbon energy status. Although glucose is present in ara1-1 similar to wild-type levels, it constitutively changes gene expression as typically found in wild-type plants only under starvation conditions. Furthermore, ara1-1 shows increased expression of marker genes for programmed cell death as found in other lesion mimic mutants |
759961 |
| 2.7.1.46 | more |
plants are knocked-out for arabinokinase by T-DNA insertion, the mutant KO plants accumulate higher levels of arabinose but do not show signs of arabinose toxicity. Creation of a double knockout plant ara1-2 ara2-1 double mutant by crossing the single knockout plants. 193fold increase of Km value for L-arabinose for ARA1-1 compared to wild-type. Infiltration of Nicotiana benthamiana leaves with ARA2 and ARA2-1 constructs showing first signs of necrosis after 3 days at the sites of infiltration. Arabinose feeding causes UDP-sugar changes in ara1-1 and wild-type (UDP-sugars: UDP-Ara, UDP-Xyl, UDP-Gal and UDP-Glc), and causes an increase in soluble carbohydrates in ara1-1 |
759961 |
| 2.7.1.46 | more |
plants are knocked-out for arabinokinase by T-DNA insertion, the mutant KO plants accumulate higher levels of arabinose but do not show signs of arabinose toxicity. Creation of a double knockout plant ara1-2 ara2-1 double mutant by crossing the single knockout plants. Infiltration of Nicotiana benthamiana leaves with ARA2 and ARA2-1 constructs showing first signs of necrosis after 3 days at the sites of infiltration |
759961 |
