EC Number   |
Protein Variants |
Reference |
|---|
    2.5.1.9 | more |
5 mutants genes cannot be expressed recombinantly in Escherichia coli: C48S, T50R, T67R, T148R, T165R |
637580 |
| 2.5.1.9 | more |
a F2DELTA deletion mutant construct has no remaining activity |
637580 |
| 2.5.1.9 | A43L |
decrease in affinity for substrate 6,7-dimethyl-8-ribityllumazine. A43L replacement causes substantial perturbation of the overall binding site topology |
687468 |
| 2.5.1.9 | H102Q |
highly reduced activity compared to the wild-type enzyme, comparison of kinetics for wild-type and mutant enzymes |
658235 |
| 2.5.1.9 | C48S |
mutation in the activity cavity, causes significant 19F NMR chemical shift modulation of trifluoromethyl derivatives of 6,7-dimethyl-8-ribityllumazine in complex with the protein. Replacement of C48 changes the electron density topology in the N-terminal substrate binding site in the vicinity of C-6 and C-7 atoms of bound ligand |
687468 |
| 2.5.1.9 | F2A |
nearly inactive mutant, comparison of kinetics for wild-type and mutant enzymes |
658235 |
| 2.5.1.9 | more |
overexpression in Escherichia coli can suppress the lack of pyrimidine deaminase/reductase in a riboflavin-deficient ribD- strain |
684754 |
| 2.5.1.9 | T50A |
production by site-directed mutagenesis, replacement of threonine residue with alanine decreases the acidity of protein-bound by 1-2 orders of magnitude |
704202 |
| 2.5.1.9 | T67A |
production by site-directed mutagenesis, replacement of threonine residue with alanine decreases the acidity of protein-bound by 1-2 orders of magnitude |
704202 |
| 2.5.1.9 | more |
recombinant sequence segment 1-97 forms a homodimer that can bind riboflavin, 6,7-dimethyl-8-ribityllumazine, and trifluoromethyl-substituted 8-ribityllumazine derivatives, and is required for ligand binding, recombinant sequence segment 101-213 is unstable and only partially involved in riboflavin binding |
637583 |
