EC Number   |
Protein Variants |
Reference |
|---|
   2.5.1.69 | H78N |
site-directed mutagenesis, inactive mutant |
737452 |
| 2.5.1.69 | more |
chimera c98f, constructed by replacing the first 98 residues in Artemisia tridentata ssp. spiciformis farnesyl diphosphate synthase with the corresponding sequence from Artemisia tridentata chrysanthemyl diphosphate synthase is able to produce a 6:5 mixture of (R)-lavandulol and (R)-maconelliol and a small amount of planococcyl alcohol in presence of alkaline phosphatase. Chimeric proteins constructed from farnesyl diphosphate synthase, which catalyzes chain elongation, and chrysanthemyl diphosphate synthase, which catalyzes cyclopropanation, catalyze all four of the known isoprenoid coupling reactions to give a mixture of geranyl diphosphate by chain elongation, chrysanthemyl diphosphate by cyclopropanation, lavandulyl diphosphate by branching, and maconelliyl and planococcyl diphosphate by cyclobutanation |
687301 |
| 2.5.1.69 | more |
expression of truncated proteins with 30, 50, or 55 N-terminal residues deleted. The DELTA30- and DELTA50 proteins are unstable |
758641 |
| 2.5.1.69 | W100V |
site-directed mutagenesis, the mutant shows 15% of wild-type activity |
737452 |
| 2.5.1.69 | Y139F |
site-directed mutagenesis, the mutant shows 62% of wild-type activity |
737452 |
