EC Number   |
Protein Variants |
Reference |
|---|
   2.5.1.59 | D140N |
alpha-subunit, increased Km |
636546 |
| 2.5.1.59 | H145D |
alpha-subunit, increased Km |
636546 |
| 2.5.1.59 | H216D |
beta-subunit, KM of geranylgeranyl diphosphate increased 12fold |
636546 |
| 2.5.1.59 | Kbeta311A |
mutation decreases prenylation rate constant in absence of Mg2+. Addition of Mg2+ increases geranylgeranylation rate constant |
659363 |
| 2.5.1.59 | Kbeta311D |
mutation decreases prenylation rate constant in absence of Mg2+. Addition of Mg2+ increases geranylgeranylation rate constant |
659363 |
| 2.5.1.59 | more |
cal1-1 mutation preferentially affects Rho1p geranylgeranylation, and it can be suppressed by Rho1p overexpression, the cdc43-5 mutation in the same gene causes accumulation of Cdc42p in soluble form and can be suppressed by Cdc42p overproduction |
636547 |
| 2.5.1.59 | more |
comparison of substrate specificity, using GST-CAIL and geranylgeranyl diphosphate: best substrate of wild-type enzyme, using GST-CVIM and geranylgeranyl diphosphate: best substrate of mutant beta H216D, using GST-CAIL and farnesyl diphosphate: best substrate of mutant betaR166I |
636546 |
| 2.5.1.59 | more |
Cwg2-1 mutant has a defect in the actin organization, this mutant is identified as a single nucleotide change causing an A202T substitution in a residue conserved among the beta-subunit of enzyme, the deletion of cwg2 causes cell death |
636549 |
| 2.5.1.59 | more |
development of GGTase-I variants with expanded/altered substrate selectivity by site-directed mutagenesis |
737695 |
| 2.5.1.59 | more |
generation of several knockout lines, knockout of PGGT activity in Ppggb, phenotypes, overview |
739309 |
