EC Number   |
Protein Variants |
Reference |
|---|
    2.5.1.17 | A134L |
site-directed mutagenesis, the mutant shows unaltered activity compared to the wild-type enzyme |
674510 |
| 2.5.1.17 | C119Y |
wild-type kinetics, decreased adenosylcobalamin production in vivo, impaired protein folding leads to degradation and, thus, low expression, no rescue of ATR-deficient Salmonella strain BE620 |
690933 |
| 2.5.1.17 | C189Y |
inactive in vitro (10fold excess of substrate compared to standard), decreased adenosylcobalamin production in vivo, impaired protein folding leads to degradation and, thus, low expression (but can be purified), no rescue of ATR-deficient Salmonella strain BE620 |
690933 |
| 2.5.1.17 | C79A |
site-directed mutagenesis of EutT, the mutant shows highly reduced activity compared to the wild-type enzyme |
674576 |
| 2.5.1.17 | C79A |
the mutant shows 1 order of magnitude reduced catalytic efficiency compared to the wild type enzyme |
-, 738536 |
| 2.5.1.17 | C80A |
inactive |
-, 738536 |
| 2.5.1.17 | C80A |
site-directed mutagenesis of EutT, the mutant shows 99% reduced activity compared to the wild-type enzyme |
674576 |
| 2.5.1.17 | C83A |
site-directed mutagenesis of EutT, the mutant shows 99% reduced activity compared to the wild-type enzyme |
674576 |
| 2.5.1.17 | C83A |
the mutant shows 30fold reduced catalytic efficiency compared to the wild type enzyme |
-, 738536 |
| 2.5.1.17 | D110R |
no activity detected with the mutant enzyme |
-, 758827 |
