EC Number |
Protein Variants |
Reference |
---|
2.4.1.B72 | E206N |
site-directed mutatgenesis, the mutation of DPY19L3, resulting in the N-glycosylation consensus sequence (206NFT208), does not induce N-glycosylation, confirming the region is certainly a cytoplasmic region |
759174 |
2.4.1.B72 | more |
CRISPR/Cas9 technology is used to generate a Pf DPY19 null mutant. Pf DPY19 gene disruption is not associated with a growth phenotype, not even under endoplasmic reticulum-stressing conditions that could impair protein folding |
759899 |
2.4.1.B72 | more |
DPY19L3 has a splice variant, called isoform2, which lacks exons 16-19 and retains part of the intron sequence, resulting in a 542-amino-acid protein. Based on our topological model, isoform2 lacks the C-terminal luminal region of DPY19L3. Topological prediction of isoform2 is almost similar to wild-type DPY19L3, but the last transmembrane (TM) region of wild-type DPY19L3 is changed to re-entrant loop (RL), resulting in the C-terminus of isoform2 is predicted to localize cytoplasm. By redox-sensitive luciferase assay, the C-terminus of isoform2 has high Gaussia luciferase activity, as does this segment of DPY19L3, demonstrating the C-terminus of isoform2 is localized to ER lumen. Furthermore, isoform2 is localized in the ER similar to wild-type DPY19L3 |
759174 |
2.4.1.B72 | more |
enzyme knockdown by siRNA |
736780 |
2.4.1.B72 | N118Q |
site-directed mutagenesis of the N-glycosylation site |
759174 |
2.4.1.B72 | N118Q/N704Q |
site-directed mutagenesis of N-glycosylation sites |
759174 |
2.4.1.B72 | N319Q |
site-directed mutagenesis of a potential N-glycosylation site |
759174 |
2.4.1.B72 | N439Q |
site-directed mutagenesis of a potential N-glycosylation site |
759174 |
2.4.1.B72 | N704Q |
site-directed mutagenesis of the N-glycosylation site |
759174 |