EC Number |
Protein Variants |
Reference |
---|
2.4.1.212 | C117F |
site-directed mutagenesis, no expression in yeast possible |
659175 |
2.4.1.212 | C117L |
site-directed mutagenesis, reduced activity compared to the wild-type enzyme |
659175 |
2.4.1.212 | C117S |
site-directed mutagenesis, activity is similar to the wild-type enzyme |
659175 |
2.4.1.212 | C210S |
site-directed mutagenesis, reduced activity compared to the wild-type enzyme |
659175 |
2.4.1.212 | C226A |
site-directed mutagenesis, 24% remaining activity and altered kinetic constants compared to the wild-type enzyme |
659171 |
2.4.1.212 | C226A |
site-directed mutagenesis, increased sensitivity to inhibition by NEM |
658855 |
2.4.1.212 | C226A |
site-directed mutagenesis, the mutant shows 44% of wild-type activity |
722359 |
2.4.1.212 | C226A/C262A |
site-directed mutagenesis, 3.2% remaining activity and altered kinetic constants compared to the wild-type enzyme |
659171 |
2.4.1.212 | C226A/C262A |
site-directed mutagenesis, slightly increased sensitivity to inhibition by NEM, and reduced sensitivity to inhibition by sodium arsenite compared to the wild-type enzyme |
658855 |
2.4.1.212 | C226A/C262A |
site-directed mutagenesis, the mutant shows 36% of wild-type activity, mutant kinetics compared to the wild-type enzyme |
722359 |