EC Number   |
Protein Variants |
Reference |
|---|
   2.4.1.144 | D321A |
inactive |
722343 |
| 2.4.1.144 | D321A |
site-directed mutagenesis, expression level in COS-1 cells similar to wild-type, but no remaining catalytic activity |
638229 |
| 2.4.1.144 | D323A |
expression of a catalytically inactive D232A mutant in human hepatoblastoma cell line Huh6, leading to suppression of the endogenous enzyme activity, but not to a significant decrease in the expression level of the endogenous enzyme |
638229 |
| 2.4.1.144 | D323A |
site-directed mutagenesis, expression level in COS-1 cells similar to wild-type, but no remaining catalytic activity |
638229 |
| 2.4.1.144 | D329A |
site-directed mutagenesis, unaltered compared to wild-type |
638229 |
| 2.4.1.144 | more |
gene mgat3 suppression via shRNA. A significant increase in ovarian masses and metastasis in control shRNA cells compared with GnT-III shRNA cells is observed. Control cell tumors are more invasive following i.p. injections in NOD/SCID mice or human carcinoma samples, whereas GnT-III shRNA tumors are less vascularized and never invade surrounding tissues |
759483 |
| 2.4.1.144 | more |
GnT-III overexpression does not affect cell morphology. Gastric cells overexpressing GnT-III appears to exhibit a more focused immunolocalization at cell-cell borders than control cells. In GnT-III transfected cells there is a well-localized continuous staining of E-cadherin at the cell membrane that correlates with F-actin staining, suggesting co-localization, GnT-III overexpression prevents clathrin-dependent E-cadherin endocytosis, phenotype, overview |
721738 |
| 2.4.1.144 | more |
overexpression of GnT-III significantly inhibits alpha2,3-sialylation but not alpha2,6-sialylation. The migratory ability of cells, HeLa-S3, CHO-K1 and Pro-5, without or with a low level of alpha2,6-sialylation is consistently suppressed after overexpression of GnT-III, while in alpha2,6-hypersialylated MDA-MB-231 and MKN-45 cells little effects are observed. Phenotypes, detailed overview |
736507 |
| 2.4.1.144 | more |
WM-266-4-GnT-III human metastatic melanoma cells stably overexpress the MGAT3 gene together with mock control cells WM-266-4-pIRESneo |
759316 |
| 2.4.1.144 | N243Q |
- |
638228 |
