EC Number |
Protein Variants |
Reference |
---|
2.4.1.101 | C121A |
active mutant enzyme. After 1 h, the wild-type MBP-hGnT1(D103) and C121A mutant convert more than 50% of Man5-RNAse B to RNAse B-GlcNAc2-Man5-GlcNAc |
684869 |
2.4.1.101 | C121D |
mutant enzyme shows no transfer of GlcNAc from UDP-GlcNAc to the glycoprotein acceptor Man5-RNAse B |
684869 |
2.4.1.101 | C121S |
active mutant enzyme. After 1 h, the C121S mutant shows less than 25% conversion of Man5-RNAse B to RNAse B-GlcNAc2-Man5-GlcNAc |
684869 |
2.4.1.101 | C121T |
mutant enzyme shows no transfer of GlcNAc from UDP-GlcNAc to the glycoprotein acceptor Man5-RNAse B |
684869 |
2.4.1.101 | C123R |
mutant enzyme does not show any activity in an HPLC analysis |
671754 |
2.4.1.101 | C907T |
point mutation in mutant cell line Lec1A.3E and Lec1A.5J, altering interactions important for stabilization of the structure, correction of the mutation by site-directed mutagenesis restores enzyme activity |
488380 |
2.4.1.101 | D144N |
D144N muation in the GnTI of cgl mutant lacking GnTI activity is molecular basis of the defect. The mutation creates an additional N-glycosylation site, which interferes with the proper folding of the enzyme |
657862 |
2.4.1.101 | D144N |
expression of D144N muatant enzyme of rabbit in cgl mutant of Arabidopsis thaliana lacking GnTI activity (caused by a D144N mutation in GnTI) can partially restore complex N-glycan formation |
657862 |
2.4.1.101 | D291N |
inactive |
721884 |
2.4.1.101 | D77N |
exchange mutation in chimera TfR/GnT1myc, no effect on Golgi localization and inclusion into high molecular weight complexes, no catalytic activity |
488377 |