EC Number |
Protein Variants |
Reference |
---|
2.3.1.269 | C23A/C62A |
site-directed mutagenesis |
757766 |
2.3.1.269 | C387A |
inactive |
747066, 748746 |
2.3.1.269 | C387A |
the mutation affects the N-acylation activity of the enzyme |
748078 |
2.3.1.269 | C387S |
inactive |
747066, 748683, 749188 |
2.3.1.269 | C387S |
site-directed mutagenesis, the mutant catalyzes the 1st step of the N-acyl transfer reaction and forms a oxygen-ester acyl intermediate but is unable to transfer the acyl group onto apolipoprotein |
758448 |
2.3.1.269 | E267A |
the mutation affects the N-acylation activity of the enzyme |
748078 |
2.3.1.269 | E343A |
the mutation affects the N-acylation activity of the enzyme |
748078 |
2.3.1.269 | E389A |
site-directed mutagenesis, a Nit domain residue, the mutant cannot complement enzyme-deficient mutant DELTAlnt cells |
757766 |
2.3.1.269 | E389A |
the mutation affects the N-acylation activity of the enzyme |
748078 |
2.3.1.269 | F358V |
inactive |
748683 |