EC Number   |
Protein Variants |
Reference  |
|---|
    2.3.1.24 | H212A |
site-directed mutagenesis |
736471 |
| 2.3.1.24 | more |
enzyme knockout by shRNA |
736471 |
| 2.3.1.24 | more |
no loss of activity is observed for the unglycosylated mutant (HA-tagged Lass6-N18Q) when compared with either the glycosylated mutant HA-tagged Lass6-N285Q or the glycosylated wild-type Lass6 |
752700 |
| 2.3.1.24 | N18Q |
site-directed mutagenesis, a glycosylation site mutant |
752700 |
| 2.3.1.24 | N285Q |
site-directed mutagenesis, a putative glycosylation site mutant |
752700 |
| 2.3.1.24 | more |
complementation of the growth defect of DELTAlag1/DELTAlac1 yeast deletion mutant by recombinant GST-/FLAG-tagged LOH2. Complementation with LOH2 leads to the accumulation of C16-containing inositolphosphoceramides and ceramides indicating this isoform's preference for C16 fatty acids. Microsomes isolated from LOH2 overexpressing plants showed high levels of C16-ceramide synthase activity compared to microsomes from wild-type plants, indicating that LOH2 overexpression results in accumulation of functional enzyme |
752718 |
| 2.3.1.24 | more |
in HEK-293 cells, ectopically expressed murine CerS5 only sensitized cells to doxorubicin and vincristine |
752796 |
| 2.3.1.24 | more |
CerS6 knockdown by shRNA |
753223 |
| 2.3.1.24 | H215D |
site-directed mutagenesis, the point mutation, changing a highly conserved histidine 215 into glutamate, in the Lag1 motif, which inhibits ceramide synthase function in the Lass1 and 5. With schlankH215D, an increase in ceramide levels cannot be observed |
753417 |
| 2.3.1.24 | more |
construction of larvae carrying transgenic UASschlankRNAi or UASschlankHA in combination with the hsGAL4 driver line. A short heat shock (1 h) induces schlank RNAi knockdown or schlank overexpression. Modulation of schlank activity correlates with the rate of ceramide de novo synthesis |
753417 |
