EC Number |
Protein Variants |
Reference |
---|
2.3.1.226 | H229A |
forms the C6 epimers (6R):(6S) of (2S,5S)-6-methyl-5-carboxymethylproline in the ratio of 75:25 compared to wild-type ratio of 55:45 |
723252 |
2.3.1.226 | H229A |
the CarB variant is able to form (2S,7S)-7-(carboxymethyl)-azepane-2-carboxylic acid from malonyl-CoA and L-aminopimelate semialdehyde in a yield of about 3 times higher than that obtained with wild-type CarB |
722026 |
2.3.1.226 | M108A |
forms the C6 epimers (6R):(6S) of (2S,5S)-6-methyl-5-carboxymethylproline in the ratio of 45:55 compared to wild-type ratio of 55:45 |
723252 |
2.3.1.226 | M108I |
forms the C6 epimers (6R):(6S) of (2S,5S)-6-methyl-5-carboxymethylproline in the ratio of 92:8 compared to wild-type ratio of 55:45 |
723252 |
2.3.1.226 | M108L |
forms the C6 epimers (6R):(6S) of (2S,5S)-6-methyl-5-carboxymethylproline in the ratio of 47:53 compared to wild-type ratio of 55:45. Forms the C6 epimers (6R):(6S) of (2S,5S)-6-ethyl-5-carboxymethylproline in the ratio of 72:18 compared to wild-type ratio of 65:35. Forms the C7 epimers (7R):(7S) of 7-methyl-(2S,6S)-carboxymethylpipecolic acid in the ratio of 60:40 compared to the wild-type ration of 66:34 |
723252 |
2.3.1.226 | M108V |
forms the C6 epimers (6R):(6S) of (2S,5S)-6-methyl-5-carboxymethylproline in the ratio of 95:5 compared to wild-type ratio of 55:45 |
723252 |
2.3.1.226 | M108V |
mutant is able to convert (2S)-methylmalonyl-CoA/L-glutamate semialdehyde/5-hydroxyproline/pyrroline-5-carboxylate to (6R)-6-methyl-(2S,5S)-5-carboxymethylproline |
735951 |
2.3.1.226 | Q111N |
forms the C6 epimers (6R):(6S) of (2S,5S)-6-methyl-5-carboxymethylproline in the ratio of 70:30 compared to wild-type ratio of 55:45 |
723252 |
2.3.1.226 | W79A |
forms the C6 epimers (6R):(6S) of (2S,5S)-6-methyl-5-carboxymethylproline in the ratio of 16:84 compared to wild-type ratio of 55:45. Forms the C6 epimers (6R):(6S) of (2S,5S)-6-ethyl-5-carboxymethylproline in the ratio of 12:88 compared to wild-type ratio of 65:35. Forms the C7 epimers (7R):(7S) of 7-methyl-(2S,6S)-carboxymethylpipecolic acid in the ratio of 1:99 compared to the wild-type ration of 66:34 |
723252 |
2.3.1.226 | W79F |
forms the C6 epimers (6R):(6S) of (2S,5S)-6-methyl-5-carboxymethylproline in the ratio of 17:83 compared to wild-type ratio of 55:45. Forms the C6 epimers (6R):(6S) of (2S,5S)-6-ethyl-5-carboxymethylproline in the ratio of 32:68 compared to wild-type ratio of 65:35. Forms the C7 epimers (7R):(7S) of 7-methyl-(2S,6S)-carboxymethylpipecolic acid in the ratio of : compared to the wild-type ration of 17:83 |
723252 |