EC Number |
Protein Variants |
Reference |
---|
2.1.1.41 | more |
natural amphotericine B-resistant mutant strain |
485329 |
2.1.1.41 | D65L |
potentially responsible for substrate binding to leucine by site directed mutagenesis, unchanged activity |
485340 |
2.1.1.41 | D79L |
potentially responsible for substrate binding to leucine by site directed mutagenesis, not decreased activity, 1 new activity to form 24-methylzymosterol from zymosterol |
485340 |
2.1.1.41 | E64L |
potentially responsible for substrate binding to leucine by site directed mutagenesis, unchanged activity |
485340 |
2.1.1.41 | E68L |
potentially responsible for substrate binding to leucine by site directed mutagenesis, no activity |
485340 |
2.1.1.41 | E82L |
potentially responsible for substrate binding to leucine by site directed mutagenesis, not decreased activity, 1 new activity to form 24-methylzymosterol from zymosterol |
485340 |
2.1.1.41 | E98L |
potentially responsible for substrate binding to leucine by site directed mutagenesis, unchanged activity |
485340 |
2.1.1.41 | D125L |
inactive mutant |
658072 |
2.1.1.41 | D152L |
inactive mutant |
658072 |
2.1.1.41 | D189L |
the ratio of turnover-number to KM-value for zymosterol is 1.4fold lower than the wild-type ratio |
658072 |