EC Number |
Protein Variants |
Reference |
---|
1.4.1.4 | C141S |
reductive amination activity of C141S alone is insensitive to treatment with 2-hydroxyethyl disulfide |
743643 |
1.4.1.4 | C321A |
site-directed mutagenesis, the mutant shows reduced NADP-related GDH activity, the mutation causes a greater than a 2fold drop in Vmax and more than a 2fold increase in Km for NADP+ |
762699 |
1.4.1.4 | C415S |
mutant loses its reductive amination activity in a manner very similar to the native enzyme |
743643 |
1.4.1.4 | D167T |
the mutant enzyme is slightly more thermostable than the wild-type enzyme |
-, 728754 |
1.4.1.4 | E158Q |
3.3% as active as wild-type |
391646 |
1.4.1.4 | K110L |
a naturally occurring mutation responsible for the inactivation of the catalytic site of Gdh1 |
-, 763438 |
1.4.1.4 | K116A |
almost complete loss of activity |
-, 741821 |
1.4.1.4 | K128A |
almost complete loss of activity |
-, 741821 |
1.4.1.4 | K136A |
residue is directly involved in binding the 2'-phosphate group of NADP+, increase in Km value for NADPH, fourfold increase in the Km value for NADH with a concomitant 1.6fold increase in the kcat value |
-, 742528 |
1.4.1.4 | K286Q |
site-directed mutagenesis, the mutant shows increased KM for NADP+ compared to the wild-type enzyme |
724981 |