EC Number |
Protein Variants |
Reference |
---|
1.3.99.4 | S325F |
mutant enzyme has no detectable KSTD enzyme activity |
-, 656722 |
1.3.99.4 | T503I |
mutant enzyme has no detectable KSTD enzyme activity |
-, 656722 |
1.3.99.4 | more |
targeted disruption of the MSMEG5898 (ksdD-1) gene, but not the MSMEG4855 (ksdD-2) gene, results in partial inactivation of the cholesterol degradation pathway and accumulation of the intermediate 4-androstene-3,17-dione, reversible by the introduction of the wild-type ksdD-1 gene into deltaksdD-1 or overexpression of ksdD-2 |
-, 675829 |
1.3.99.4 | Y119F |
almost complete loss of activity |
-, 742850 |
1.3.99.4 | Y318F |
almost complete loss of activity |
-, 742850 |
1.3.99.4 | Y487F |
almost complete loss of activity |
-, 742850 |
1.3.99.4 | more |
enzyme from strain ST-095 only differs in residue V366 from strain JC-12 (S366). Mutation results in almost 3fold higher catalytic efficiency in strain JC-12 |
-, 743031 |
1.3.99.4 | A255F |
site-directed mutagenesis, inactive mutant |
763180 |
1.3.99.4 | A255G |
site-directed mutagenesis, inactive mutant |
763180 |
1.3.99.4 | F266A |
site-directed mutagenesis, inactive mutant |
-, 763180 |