EC Number   |
Protein Variants |
Reference |
|---|
    1.3.1.75 | more |
a strain of Rhodobacter sphaeroides mutant lacking BchJ and BciA with incorporated bchYZ of bacteriochlorophyll b-producing Blastochloris viridis, encoding chlorophyllide a oxidoreductase (COR), shows accumulating of both BChla and BChlb and becomes an anoxygenic photosynthetic bacterium producing BChls a and b together. Loss of functions of both intrinsic COR and 8-vinyl reductase (BciA) in the host and expression of the BchYZ catalytic components of COR from Blastochloris viridis, not the complete set of COR (BchXYZ), is essential for production of bacteriochlorophylls a and b in the host strain |
746442 |
| 1.3.1.75 | more |
BciA and BciB can be readily exchanged, and BciA can be efficiently transferred to cyanobacteria |
762685 |
| 1.3.1.75 | more |
construction of a bchXYZ deletion mutant, construction of a Rhodobacter palustris bciA mutant. The mutant lacking sole BciA can grow under anaerobic light conditions as well as aerobic dark conditions. On the other hand, the single bchXYZ mutant and the double bciA/bchXYZ mutant do not show phototrophic growth and grow under aerobic/semiaerobic conditions in the dark |
-, 736444 |
| 1.3.1.75 | more |
construction of a bchXYZ deletion mutant, construction of a Rhodobacter palustris bciB mutant using the plasmid pJSC-palB-Km |
-, 736444 |
| 1.3.1.75 | more |
construction of mutant tepdA lacking enzymes BciA and BchU, that catalyze reduction of the C8-vinyl group and methylation at the C20 position of bacteriochlorophyll (BChl) c, respectively, in the green sulfur bacterium Chlorobaculum tepidum, the mutant accumula C8-vinyl-bacteriochlorophyllide d derivative, determined by NMR to be (31R)-8-vinyl-12-ethyl-(R[V,E])BChl d. The bciA mutant possesses mainly (31R)-8-vinyl-12-ethyl-(R[V,E])BChl c as in chlorosomal self-aggregates in addition to minor (31R)-8-vinyl-12-methyl-(R[V,M]) and (31S)-8-vinyl-12-ethyl-(S[V,E])BChls c. Reconstitution of self-aggregates of C8V-BChl species in vitro. Comparison of chlorophyllide a derivatives in tepdA strains lacking bciA and/or bciU, overview |
-, 745981 |
| 1.3.1.75 | more |
engineered pathway design for the heterologous production of bacteriochlorophyll in the non-photosynthetic host Escherichia coli expressing the enzyme involved originating from different organisms, overview. CTBciA is capable of reducing the C8-vinyl group of several different intermediates in the BChl pathway. No mono-vinyl forms of any of the pathway intermediates upon coexpression of the 8-vinyl reductase with BchSID and BchM. PIX overproducing cells expressing BchSID and CTBciA alone produce two additional compounds, mono-vinyl PIX (mvPIX) and mono-vinyl MgPIX (mvMgPIX), indicating that CTBciA is capable of reducing the C8-vinyl group on both the Mg chelated and the non-Mg chelated porphyrin molecule |
-, 746260 |
| 1.3.1.75 | more |
engineered pathway design for the heterologous production of bacteriochlorophyll in the non-photosynthetic host Escherichia coli expressing the enzyme involved originating from different organisms, overview. RSBciA is completely inactive in our recombinant system. RSBciA is completely inactive in our recombinant system. Subcloning in Escherichia coli strain JM109 |
746260 |
| 1.3.1.75 | more |
generation of the OsDVR-inactivated 824ys mutant of rice that shows no DVR activity |
746105 |
