EC Number |
Protein Variants |
Reference |
---|
1.14.99.52 | E176A |
site-directed mutagenesis, the mutant shows reduced enzyme activity by at least 100fold compared to the wild-type enzyme |
-, 734024 |
1.14.99.52 | E176H |
site-directed mutagenesis |
-, 734828 |
1.14.99.52 | E176H |
site-directed mutagenesis, the mutation changes OvoA from a 2-His-1-carboxylate to a 3-His ligand environment if the new third histidine residue replaces glutamate as the metal ligand. The mutant enzyme not only loses the cysteine dioxygenase activity but also stops catalyzing the formation of oxidative coupling products S-(L-histidin-5-yl)-L-cysteine S-oxide and cysteine sulfinic acid. The 2-His-1-carboxylate catalytic triad disrupts the cysteine dioxygenase activity |
745960 |
1.14.99.52 | H170A |
site-directed mutagenesis, the mutant shows reduced enzyme activity by at least 100fold compared to the wild-type enzyme |
-, 734024 |
1.14.99.52 | H174A |
site-directed mutagenesis, the mutant shows reduced enzyme activity by at least 100fold compared to the wild-type enzyme |
-, 734024 |