   1.14.19.36 | more |
construction of a insertional mutant fad8i (SALK_093590) inactivation mutant of FAD8, analysis of the fatty acid composition of the fad8i mutant shows compensatory responses at the trienoic fatty acid level as a result of inactivation of the FAD8 gene. Genomic analysis of fad8i plants show insertion of an inverted tandem T-DNA 133 bp downstream of the start codon, within exon 1 of the AtFAD8 gene, absence of AtFAD8 transcript in the mutant. Contruction of a fad7/fad8 double knockout mutant. Given the strong reduction (70%) in 18:3 content observed in the fad7/fad8 double mutant, in mutant fad7i after disruption of the AtFAD7 gene, FAD8 enzymatic activity is able to maintain, at least partially (43.7%), the amount of 18:3 and to a much lesser extent that of 16:3 (23.2%) at 22°C. Mutant genotypes and phenotypes, overview. Temperature-dependent expression levels. Disruption of the AtFAD8 gene in the insertion mutant does not result in significant changes in the transcript levels of FAD8. Overexpression of FAD8 increased 18:3 levels in phospholipids but not in galactolipids |
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