EC Number   |
Protein Variants |
Reference |
|---|
    1.14.15.35 | A245S |
about 10fold decrease in affinity for 6-deoxyerythronolide B, and 6fold decrease in specific affinity |
728805 |
| 1.14.15.35 | A245S |
activity is decreased by 84%, the active site structure including water is essentially unchanged with the exception that the OH group of Ser245 points toward the I-helix cleft to make new H-bonds with water 63 and the carbonyl group of the Ala241 |
-, 725366 |
| 1.14.15.35 | A245S |
significantly increased activity with substrates testosterone and 7-benzyloxyquinoline. Contrary to wild-type, binding to inhibitor ketoconazole follows type II |
-, 727300 |
| 1.14.15.35 | A245T |
about 10fold decrease in affinity for 6-deoxyerythronolide B, and 1000fold decrease in specific affinity |
728805 |
| 1.14.15.35 | A245T |
increased activity with substrate 7-benzyloxyquinoline. Like wild-type, type-II binding to inhibitor ketoconazole |
-, 727300 |
| 1.14.15.35 | A245T |
more than 99% loss of activity |
-, 725366 |
| 1.14.15.35 | more |
construction of an erythronolide H-producing Saccharopolyspora erythraea mutant via gene complementation is not successful. Construction of en eryF deletion mutant strainEX105 from strain ZL2001 and complementation by Actinopolyspora erythraea eryF expression |
-, 746251 |
