EC Number |
Protein Variants |
Reference |
---|
1.14.11.43 | E69A |
exhibits 30.8% of wild-type enzyme activity with (S)-2-(4-chloro-2-methylphenoxy)propanoate. kcat/Km is 42% compared to the wild-type value |
723719 |
1.14.11.43 | G97I/N98G |
exhibits 3.8% of wild-type enzyme activity with (S)-2-(4-chloro-2-methylphenoxy)propanoate. kcat/Km is 0.6% compared to the wild-type value |
723719 |
1.14.11.43 | G97N/N98G |
exhibits 0.3% of wild-type enzyme activity with (S)-2-(4-chloro-2-methylphenoxy)propanoate |
723719 |
1.14.11.43 | H208A |
exhibits about 1.4% of wild-type enzyme activity with (S)-2-(4-chloro-2-methylphenoxy)propanoate. kcat/Km is 0.1% compared to the wild-type value |
723719 |
1.14.11.43 | H272A |
exhibits about 1.2% of wild-type enzyme activity with (S)-2-(4-chloro-2-methylphenoxy)propanoate |
723719 |
1.14.11.43 | Q162F |
exhibits about 4.8% of wild-type enzyme activity with (S)-2-(4-chloro-2-methylphenoxy)propanoate |
723719 |
1.14.11.43 | R207A |
exhibits about 17.3% of wild-type enzyme activity with (S)-2-(4-chloro-2-methylphenoxy)propanoate. kcat/Km is 2% compared to the wild-type value |
723719 |
1.14.11.43 | R207V |
exhibits about 2.8% of wild-type enzyme activity with (S)-2-(4-chloro-2-methylphenoxy)propanoate |
723719 |
1.14.11.43 | R274A |
inactive mutant enzyme |
723719 |