EC Number   |
Protein Variants |
Reference |
|---|
   1.13.11.B6 | F580A |
kcat/KM for linoleate hydroperoxidation is increased by 35% |
703535 |
| 1.13.11.B6 | L569V |
kcat/KM for linoleate hydroperoxidation is reduced by 38% |
703535 |
| 1.13.11.B6 | T579F |
kcat/KM for linoleate hydroperoxidation is reduced by 89% |
703535 |
| 1.13.11.B6 | W523A |
kcat/KM for linoleate hydroperoxidation is reduced by 98% |
703535 |
| 1.13.11.B6 | I437L |
KM-value for linoleate is 114% of wild-type value, kcat/Km for linoleate is 83% of wild-type value. Positional specificity 13-hydroperoxy-(10E,12Z)-octadecadienoate/9-hydroperoxy-(10E,12Z)-octadecadienoate is 0.7 (compared to 0.8 in reaction with wild-type enzyme) |
396190 |
| 1.13.11.B6 | V580F |
KM-value for linoleate is 384% of wild-type value, kcat/Km for linoleate is 4% of wild-type value. Positional specificity 13-hydroperoxy-(10E,12Z)-octadecadienoate/9-hydroperoxy-(10E,12Z)-octadecadienoate is 0.9 (compared to 0.8 in reaction with wild-type enzyme) |
396190 |
| 1.13.11.B6 | V570I |
KM-value for linoleate is 61% of wild-type value, kcat/Km for linoleate is 367% of wild-type value. Positional specificity 13-hydroperoxy-(10E,12Z)-octadecadienoate/9-hydroperoxy-(10E,12Z)-octadecadienoate is 0.6 (compared to 0.8 in reaction with wild-type enzyme) |
396190 |
| 1.13.11.B6 | more |
mutants Arg268Ala, Gly567Ala, Ile578Leu and Val582Phe show high substrate conversion rates of linoleate, linolenate and arachidonate (60%-100% of wild-type LOX1:Md:1a activity with linoleate). Substrate specificity of mutants, overview |
742627 |
| 1.13.11.B6 | F580A |
possesses activity profile that is similar to wild-type |
703535 |
| 1.13.11.B6 | F580V |
possesses activity profile that is similar to wild-type |
703535 |
