EC Number |
Protein Variants |
Reference |
---|
1.13.11.6 | E110A |
kcat/Km is 954fold lower than wild-type enzyme |
672037 |
1.13.11.6 | H52A |
inactive enzyme |
-, 649382 |
1.13.11.6 | H93A |
24.8% activity of the native enzyme |
-, 649382 |
1.13.11.6 | H96A |
inactive enzyme |
-, 649382 |
1.13.11.6 | I142A |
the variant cannot form a closed conformation and has a substantially higher Km for O2 than wild-type enzyme |
-, 765043 |
1.13.11.6 | I142P |
the variant cannot form a closed conformation and has a substantially higher Km for O2 than wild-type enzyme |
-, 765043 |
1.13.11.6 | N27A |
the variant cannot form a closed conformation and has a substantially higher Km for O2 than wild-type enzyme |
-, 765043 |
1.13.11.6 | Q105A |
site-directed mutagenesis, the mutation affects the enzyme activity, the protein structure, particularly the active site architecture and the metal ion environment, and the substrate binding |
743276 |
1.13.11.6 | R43A |
site-directed mutagenesis, the mutation affects the enzyme activity, the protein structure, particularly the active site architecture and the metal ion environment, and the substrate binding |
743276 |
1.13.11.6 | R47A |
kcat/Km is 7440fold lower than wild-type enzyme. Mutant enzyme shows substrate inhibition |
672037 |