EC Number |
Protein Variants |
Reference |
---|
1.13.11.11 | 2 F51S/T254S |
variant synthesizes monooxigenation product 3alpha-hydroxyhexahydropyrrolo[2,3-b]indole-2-carboxylic acid in a ratio of 6.2:1 to wild-type product N-formyl-L-kynurenine |
763893 |
1.13.11.11 | 3 F51Q/G255S |
variant synthesizes monooxigenation product 3alpha-hydroxyhexahydropyrrolo[2,3-b]indole-2-carboxylic acid in a ratio of 3.1:1 to wild-type product N-formyl-L-kynurenine |
763893 |
1.13.11.11 | 4 F51M/Q127Y/T254S |
variant synthesizes monooxigenation product 3alpha-hydroxyhexahydropyrrolo[2,3-b]indole-2-carboxylic acid in a ratio of 2.6:1 to wild-type product N-formyl-L-kynurenine |
763893 |
1.13.11.11 | F140A |
site-directed mutagenesis, the mutant shows 0.25% of wild-type activity |
743760 |
1.13.11.11 | F51I/Q127Y |
mutant F51M/Q127Y shows higher 3alpha-hydroxyhexahydropyrrolo[2,3-b]indole-2-carboxylic acid productivity than mutant F51I/Q127Y and mutant F51L/Q127Y, suggesting that the F51M and Q127Y substitutions imposed a cooperative effect on the enzyme activity |
763893 |
1.13.11.11 | F51L/Q127Y |
mutant F51M/Q127Y shows higher 3alpha-hydroxyhexahydropyrrolo[2,3-b]indole-2-carboxylic acid productivity than mutant F51I/Q127Y and mutant F51L/Q127Y, suggesting that the F51M and Q127Y substitutions imposed a cooperative effect on the enzyme activity |
763893 |
1.13.11.11 | F51M/Q127Y |
mutant F51M/Q127Y shows higher 3alpha-hydroxyhexahydropyrrolo[2,3-b]indole-2-carboxylic acid productivity than mutant F51I/Q127Y and mutant F51L/Q127Y, suggesting that the F51M and Q127Y substitutions imposed a cooperative effect on the enzyme activity |
763893 |
1.13.11.11 | F51M/Q127Y |
variant synthesizes monooxigenation product 3alpha-hydroxyhexahydropyrrolo[2,3-b]indole-2-carboxylic acid in a ratio of 2.0:1 to wild-type product N-formyl-L-kynurenine |
763893 |
1.13.11.11 | F51M/Q127Y/T254S |
mutations result in a decrease in the turnover numbers for production of 3alpha-hydroxyhexahydropyrrolo[2,3-b]indole-2-carboxylic acid |
763893 |
1.13.11.11 | F51Q/G255S |
mutation enhances the 3alpha-hydroxyhexahydropyrrolo[2,3-b]indole-2-carboxylic acid/N'-formylkynurenine ratio to 3.1:1 |
763893 |