EC Number   |
Protein Variants |
Reference |
|---|
    1.1.1.34 | A333P |
mutation disrupts Insig binding and abolishes sterol-accelerated degradation. The pivotal event for sterol-induced degradation of the choletsreol biosynthetic enzyme HMG-CoA reductase is binding of its membrane domain to Insig proteins in the endoplasmic reticulum. Insig are carriers for gp78, an E3 ubiquitin ligase that marks reductase for proteasomal degradation |
669764 |
| 1.1.1.34 | G87R |
mutation disrupts Insig binding and abolishes sterol-accelerated degradation. The pivotal event for sterol-induced degradation of the choletsreol biosynthetic enzyme HMG-CoA reductase is binding of its membrane domain to Insig proteins in the endoplasmic reticulum. Insig are carriers for gp78, an E3 ubiquitin ligase that marks reductase for proteasomal degradation |
669764 |
| 1.1.1.34 | H398Q |
low activity for catalysis of reductive deacylation of (S)-3-hydroxy-3-methylglutaryl-CoA or oxidative acylation of mevaldehyde, but readily catalyzed mevaldehyde reduction |
-, 728768 |
| 1.1.1.34 | L403R/G404R/A406S |
engineering of an appropriately located phosphoacceptor serine and cAMP-dependent protein kinase recognition motif to create a phosphorylation site. Km values, Vmax, optimal pH and temperature of mutant are identical to wild-type. Exposure of mutant to ATP and cAMP-dependent protein kinase is accompanied by incorporation of phosphate and by a parallel decrease in catalytic activity. Subsequent treatment with a protein phosphatase releases enzyme-bound 32Pi and restores activity to pretreatment levels |
286586 |
| 1.1.1.34 | L403R/G404R/A406S |
regulation of activity by phosphorylation |
286586 |
| 1.1.1.34 | L498I |
the naturally occuring mutation T1564C creates a c-Rel binding site |
722328 |
| 1.1.1.34 | M430T |
naturally occuring mutation T1392C |
722328 |
| 1.1.1.34 | more |
coexpression of N-terminal truncated Hevea brasiliensis (S)-3-hydroxy-3-methylglutaryl CoA reductase and tobacco C24-sterol methyltransferase type 1 in transgenic Nicotiana tabacum plants enhances carbon flux towards end-product sterols, expression under control of both seed-specific and constitutive promotors leads to enhancement of sterol accumulation by 2.5 and 2.1fold, respectively, analysis of the sterol spectrum, overview |
657004 |
| 1.1.1.34 | more |
construction of insertion mutants of the 2 isozymes leads to loss of enzyme function, the hmg1 mutant plants show an altered phenotype with dwarfing, early senescence, male sterility, and both mutants of hmg1 and hmg2 show reduced sterol levels, the mutants are more sensitive to the inhibitor lovastatin and squalestatin, overview |
657007 |
| 1.1.1.34 | more |
expression of human HMG-CoA reductase in yeast complements the lethal phenotype of Saccharomyces cerevisiae strains lacking the HMG1 and HMG2 genes |
698528 |
