EC Number |
Protein Variants |
Reference |
---|
1.1.1.267 | D151N/E222Q |
loss of catalytic ability, loss of binding of Mn2+. Crystallization data |
-, 687567 |
1.1.1.267 | D152A |
site-directed mutagenesis, active site mutant, in sense and antisense orientation, 4.1% activity compared to the wild-type enzyme |
672396 |
1.1.1.267 | D152N |
site-directed mutagenesis, active site mutant, in sense and antisense orientation, inactive mutant |
672396 |
1.1.1.267 | E154D |
site-directed mutagenesis, active site mutant, in sense and antisense orientation, 0.28% activity compared to the wild-type enzyme |
672396 |
1.1.1.267 | E154Q |
site-directed mutagenesis, active site mutant, in sense and antisense orientation, 0.008% activity compared to the wild-type enzyme |
672396 |
1.1.1.267 | E223H |
site-directed mutagenesis, active site mutant, in sense and antisense orientation, 0.007% activity compared to the wild-type enzyme |
672396 |
1.1.1.267 | E223Q |
site-directed mutagenesis, active site mutant, in sense and antisense orientation, inactive mutant |
672396 |
1.1.1.267 | E231K |
less than 0.24% wild type kcat |
286428 |
1.1.1.267 | G14D |
mutant |
286428 |
1.1.1.267 | H153Q |
increase of Km |
286428 |