EC Number |
Protein Variants |
Reference |
---|
1.1.1.169 | K176A/E256A |
double mutant, no activity |
286109 |
1.1.1.169 | more |
generation of a gene TK1968 disruption mutant |
-, 736827 |
1.1.1.169 | more |
Lys176 acts as general acid in ketopantoate reduction and is involved in catalysis and ketopantoate binding, E256A functions in D-pantoate and ketopantoate binding in ketopantoate reductase |
286109 |
1.1.1.169 | more |
Lys176 and Glu256 important for binding ketopantoate and the catalytic mechanism |
286110 |
1.1.1.169 | C84A |
site-directed mutagenesis |
-, 739822 |
1.1.1.169 | W129A |
site-directed mutagenesis |
-, 739822 |
1.1.1.169 | Y60A |
site-directed mutagenesis |
-, 739822 |
1.1.1.169 | E256A |
site-directed mutagenesis, altered steady-state kinetics of the mutant compared to the wild-type enzyme, overview |
687675 |
1.1.1.169 | K176A |
site-directed mutagenesis, altered steady-state kinetics of the mutant compared to the wild-type enzyme, overview |
687675 |
1.1.1.169 | K72A |
site-directed mutagenesis, altered steady-state kinetics of the mutant compared to the wild-type enzyme, overview |
687675 |