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Protein Variants:
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EC Number
Protein Variants
Commentary
Reference
3.6.1.28
W53A
2.5fold increase in catalytic efficiency
733393
3.6.1.28
D37A
about 0.5% of wild-type catalytic efficiency
733393
3.6.1.28
K11A
about 15% of wild-type catalytic efficiency
733393
3.6.1.28
D147A
about 30% of wild-type catalytic efficiency
733393
3.6.1.28
E81A
about 30% of wild-type catalytic efficiency
733393
3.6.1.28
Y39A
about 30% of wild-type catalytic efficiency
733393
3.6.1.28
Y79A
about 5% of wild-type catalytic efficiency
733393
3.6.1.28
K193A
about 60% of wild-type catalytic efficiency
733393
3.6.1.28
K85E
decreased solubility, reduced catalytic activity
667797
3.6.1.28
K65A
more than 1000fold decrease in kcat value
733393
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