EC Number   |
Protein Variants |
Reference |
|---|
   2.4.1.90 | D254E |
0.01% of the activity of the wild-type enzyme |
489556 |
| 2.4.1.90 | D254N |
0.01% of the activity of the wild-type enzyme |
489556 |
| 2.4.1.90 | C342S |
33fold increase in the apparent Km-value for UDPgalactose |
489552 |
| 2.4.1.90 | more |
a construct bearing the N-terminal peptides from bovine Cd1 (residues 143175 (P1)) fused with peptide with the Cd7DELTAC protein (last 11 amino acids deleted) shows no impact on the catalytic activity nor the folding efficiency. This construct is used for successful crystallization |
719863 |
| 2.4.1.90 | DELTA1-70/DELTA312-322 |
a DNA fragment bearing the Cd7 catalytic domain and C-terminal 11-amino acid deletion (Cd7DELTAC) sequence (residues 71-311) shows no difference in catalytic activity or folding but crystalization is not possible |
719863 |
| 2.4.1.90 | C134S |
complete loss of activity |
489552 |
| 2.4.1.90 | DELTA1-70 |
crystallization of a refolded Drosophila Cd7 protein coding the catalytic domain (Cd7) sequence (residues 71-322) is not successful |
719863 |
| 2.4.1.90 | more |
decreasing the expression of beta1,4GalT II using RNA interference inhibits p53-mediated HeLa cell apoptosis induced by adriamycin |
687455 |
| 2.4.1.90 | H347D |
in presence of Mn2+ retains 0.02% of wild-type enzyme activity, in presence of Co2+ retains 0.085% of wild-type enzyme activity |
489556 |
| 2.4.1.90 | H347N |
in presence of Mn2+ retains 0.07% of wild-type enzyme activity, in presence of Co2+ retains 0.36% of wild-type enzyme activity |
489556 |
