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EC Number
Protein Variants
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5.5.1.13
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sequence contains a DVDD motif responsible for copalyl diphosphate synthase activity and a DDYFD motif responsible for ent-kaurene synthase activity. Mutation of DVDD motif to AVAD leads to loss of function, mutation of DDYFD motif to AAYFD results in accumulation of ent-copalyl diphosphate
679843
5.5.1.13
N425A
the mutant exhibits a 13fold reduction in kcat
729301
5.5.1.13
R340A
the mutant exhibits an 850fold reduction in kcat
729301
5.5.1.13
T421A
the mutant exhibits a 163fold reduction in kcat (KM is increased 2fold)
729301
5.5.1.13
T421S
the mutant exhibits a 3fold reduction in kcat
729301
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