EC Number   |
Protein Variants |
Reference |
|---|
   4.2.3.9 | V88F |
products are 18.4% aristolochene, 57.8% germacrene A, 23.8% valencene |
716415 |
| 4.2.3.9 | W334F |
ratio of products: 82% aristolochene, 18% germacrene A |
715288 |
| 4.2.3.9 | W334H |
ratio of products: 10% aristolochene, 90% germacrene A |
715288 |
| 4.2.3.9 | W334L |
ratio of products: 2% aristolochene, 98% germacrene A |
715288 |
| 4.2.3.9 | W334Y |
ratio of products: 62% aristolochene, 38% germacrene A |
715288 |
| 4.2.3.9 | Y341F |
10fold reduction in catalytic efficiency |
729580 |
| 4.2.3.9 | Y92A |
reduction of the size of the side chain of residue 92 leads to the production of the alicyclic sesquiterpenes (E)-beta- and (E,E)-alpha-farnesene. The relative amounts of linear products formed depend linearly on the size of the residues at position 92. ASY92A produces almost 80% of alicyclic sesquiterpenes and no aristolochene |
644243 |
| 4.2.3.9 | Y92A |
turnover number is approximately 2 orders of magnitude lower than the value observed for the wild-type enzyme,the mutant enzyme produces almost 80% of the alicyclic sesquiterpenes (E)-beta-farnesene and (E,E)-alpha-farnesene. The mutant also produces small amounts of additional hydrocarbons with a molecular weight of 204: alpha-selinene, beta-selinene, selina-4,11-diene, (E,Z)-alpha-farnesene, and beta-bisabolene. Km-value for trans, trans-farnesyl diphosphate is 0.0834 mM compared to 0.0023 mM for the wild-type enzyme |
644243 |
| 4.2.3.9 | Y92C |
reduction of the size of the side chain of residue 92 leads to the production of the alicyclic sesquiterpenes (E)-beta- and (E,E)-alpha-farnesene. Mutant ASY92C still produces about 6.8% of aristolochene |
644243 |
| 4.2.3.9 | Y92C |
turnover number is approximately 2 orders of magnitude lower than the value observed for the wild-type enzyme. Km-value for trans, trans-farnesyl diphosphate is 0.05027 mM compared to 0.0023 mM for the wild-type enzyme |
644243 |
