EC Number   |
Protein Variants |
Reference |
|---|
    4.2.1.125 | more |
identification of single nucleotide polymorphisms in different samples of Panax ginseng cultivar DAMAYA, specific single nucleotide polymorphisms (SNPs) of the DDS gene might be associated with the accumulation of some ginsenosides in DAMAYA ginseng and thus can be used for varietal characterization, molecular identification, qualitative evaluation of ginseng-type medicinal materials, or gene engineering and ginseng breeding. The ginsenoside contents, of such as Rf, Rg1 and F1, change with the gene mutation |
748874 |
| 4.2.1.125 | more |
metabolic engineering of Pichia pastoris strain GS115 for the high yield production of dammarenediol-II, based on the native triterpene synthetic pathway, a dammarenediol-II synthetic pathway is established in Pichia pastoris by introducing a dammarenediol-II synthase gene (PgDDS) from Panax ginseng. To increase the supply of 2,3-oxidosqualene, the ERG1 gene, which is responsible for 2,3-oxidosqualene synthesis, is coexpressed. And to reduce competition for 2,3-oxidosqualene from ergosterol biosynthesis without affecting the normal growth of Pichia pastoris, the ERG7gene, which is responsible for conversion of 2,3-oxidosqualene to lanosterol, is downregulated by replacing its native promoter with a thiamine-repressible promoter, using a marker-recycling and gene-targeting Cre- lox71/66 system, developed for Pichia pastoris. Method evaluation and optimization, overview |
748268 |
| 4.2.1.125 | W421A |
site-directed mutagenesis, the mutant shows highly decreased activity compared to the wild-type enzyme |
748118 |
| 4.2.1.125 | W538A |
site-directed mutagenesis, the mutant shows highly decreased activity compared to the wild-type enzyme |
748118 |
| 4.2.1.125 | W616A |
site-directed mutagenesis, the mutant shows highly decreased activity compared to the wild-type enzyme |
748118 |
| 4.2.1.125 | Y263A |
site-directed mutagenesis, the mutant shows highly decreased activity compared to the wild-type enzyme |
748118 |
| 4.2.1.125 | Y268A |
site-directed mutagenesis, the mutant shows highly decreased activity compared to the wild-type enzyme |
748118 |
| 4.2.1.125 | Y732A |
site-directed mutagenesis, the mutant shows highly decreased activity compared to the wild-type enzyme |
748118 |
